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metapr2 2.1.0

Released: 2023-05-16

Web application and R package

  • Only the latest version (2.1) of the database is provided.
  • Taxonomy table reflects the groups selected on the side panel

Database version 2.1

  • Metabarcodes are now assigned with PR2 version 5.0.0 (9 taxonomy levels).

metapr2 2.0.1

Released: 2023-02-17

Web application and R package

Two bugs fixed:

  • Error linked to version 4.2 of R giving error instead of warning for if when the condition a dimension > 1
  • Some long sequences where giving an error in the Query panel

metapr2 2.0.0

Released: 2022-11-23

Database

59 datasets

18 new datasets

Polar
  • Arctic - 2012 (Kilias 2020)
  • Amundsen_Sea ASPIRE cruise - 2010-2011
  • Fram Strait - 2014
  • Palmer Station Antarctic - LTER - 2014
  • Arctic and Scotian Shelf - 2009-2011
  • Baffin Bay - 2008-2018
  • Southern Ocean - 2017
  • Fram observatory 2016
  • Antarctic Peninsula - 2012-2016
Oceanic
  • Atlantic transect cruise PS113 - 2018
  • South West Pacific NIWA - 2009_2018
Coastal
  • Roscoff Astan - 2009-2011
  • Roscoff Astan - 2012-2016
  • SE Asia Tsunami deposits
  • Baltic Sea Gdansk Gulf - 2012 Hapto
  • Coral Infecting Apicomplexan
  • Zostera marina - British Columbia - 2015
  • Coral Singapore - 2018
Clustering

An option is now provided to use either all ASVs or clustered ASVs on the welcome screen. ASVs are clustered at 100% identity with VSEARCH –id 1.00 See the metaPR2 paper for more information.

It is also possible to use version 1.0 of the database by entering v1 on the welcome screen.

Web application

New Panel: Taxonomy

This new panel provides a table with all the taxa present in the current metaPR2 version with the number of ASV for each species. The table can be easily searched.

Minor changes
  • Information about database is provided on left panel

  • New button to disconnect the application and reloading.

  • Maximum number of samples for Phyloseq: 2000.

  • Taxonomy is constructed from all the samples and not only samples selected.

  • Option to use clustered ASVs in Welcome panel.

  • Barplots. The right side of the graph indicates, for each parameter range, the number of samples that fall into that range as well as the number of samples that contain the taxa selected.


metapr2 1.0.3

Released: 2022-04-30

Database

version 1.1 - 41 datasets
  • Tara Ocean V9 samples have been reprocessed using the dada2 pipeline. In version 1.0, the original swarms were used instead of ASVs.

Tabs of application

Datasets
  • Selected datasets appear first ordered by dataset_id
  • Search error fixed
Query
  • A fasta formatted sequence with header can now be used.
Download
  • The zipped file now contains a fasta file with the asv_code and the taxonomy in the header.

metapr2 1.0.2

Released: 2021-12-14

Tabs of application

Datasets
  • Settings (datasets, type of samples) can be saved and recalled
Taxonomy
  • Now more than one taxon can be selected
  • Three divisions can be removed (Fungi, Metazoa and Streptophyta)
  • Taxa (selected and excluded) can be saved and recalled
  • It is necessary to press the “Validate taxa” to replot after changing taxo selection
Treemaps
  • Color of taxa now match other panels
  • Add a treemap of ASVs number
Maps
  • Add topography
  • Add equator, tropics and polar circle
Barplots
  • Add number of samples for each bar
Diversity Alpha
  • Use Violin + Sina plot for discrete variable
  • Allow discretization of continuous variables (e.g. depth, latitude)

metapr2 1.0.1

Released: 2021-11-22

Tabs of application

Documentation
Barplots
  • Make interactive (R plotly library)
  • Add coloring by ecological function
  • Add time series

metapr2 1.0.0

Released: 2021-11-19

  • Initial release

Database

version 1.0 - 41 datasets
  • Tara Ocean V9 samples have been not been reprocessed and the original swarms are used instead.